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Dr. Peter H. Graham
439 Borlaug Hall
1991 Upper Buford Circle
St Paul, MN 55406

 
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FAQ

How can you determine that a nodule is fixing nitrogen?

Nodule Structure, Alfalfa When you cut open an effective nitrogen-fixing nodule, a significant portion of the nodule should be pink or red in color. This color is due to the presence of a hemoglobin similar to that found in blood, and also involved in oxygen supply, in this case, to the bacteria. The amount of hemoglobin present is usually closely correlated to amount of nitrogen fixed, with white or green-colored nodules usually very limited in their ability to fix nitrogen. Remember though that nodules have a finite life span (in the case of soybean estimated at 50-60 days) so that toward the end of the growing season many of the initial nodules will have already begun to senesce, and are brown or green in color, while the more active nitrogen-fixing nodules may now be located on lateral roots. Levels of nitrogen fixation achieved by legumes are reviewed in the recent papers of Unkovich and Pate (2000) and van Kessel and Hartley (2000).